Chapter 2 : Image Intensities

Posted onDecember 9, 2015May 30, 2016Leave a comment

Now that we have seen how to choose and set optimal resolutions for the the microscopy experiment, we should now discuss something about how the intensity of the image that is acquired. A word on resolution and contrast It makes sense to discuss this now because the different intensities of various regions of a sample Read More

Chapter 1 : Resolution

Posted onDecember 9, 2015May 30, 2017Leave a comment

Try to optimize parameters, instead of maximizing them. Change as little as possible between different days. Do not trust your eyes – they are the least sensitive component of a microscopy experiment. ###Chapter 1 – Spatial Resolution Formal definitions of resolution refer to imaginary objects such as infinitely small sources of light. I will avoid Read More

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Emergent Noise

a mindstream from just another statistic

Category: Imaging Guidelines

Chapter 2 : Image Intensities

Chapter 1 : Resolution